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rabbit anti rat polyclonal nox2 antibody  (Bioss)


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    Bioss rabbit anti rat polyclonal nox2 antibody
    Effect of aldosterone on the protein expression levels of (A) AIF-1, p-PI3K, p-AKT, p-mTOR, <t>NOX2</t> and Nrf2 in normal rats using western blotting. Aldosterone (0.75 µg/h subcutaneous infusion) increased the expression of (B) AIF-1, (C) p-PI3K, (D) p-AKT, (E) p-mTOR and (F) NOX2 and decreased the expression of (G) Nrf2 in normal rats, which was attenuated by spironolactone (100 mg/kg/day). *P<0.05 vs. con group, **P<0.05 vs. ald group, ***P<0.05 vs. ald + spir group. The data are presented as the mean ± standard deviation (n=3). AIF-1, allograft inflammatory factor-1; p-PI3K, phosphorylated phosphatidylinositol 3-kinase; p-AKT, phosphorylated AKT serine/threonine kinase; p-mTOR, phosphorylated mammalian target of rapamycin; NOX2, NADPH oxidase 2; Nrf2, nuclear transcription factor erythroid-related factor 2; con, control; ald, aldosterone; spir, spironolactone.
    Rabbit Anti Rat Polyclonal Nox2 Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti rat polyclonal nox2 antibody/product/Bioss
    Average 94 stars, based on 36 article reviews
    rabbit anti rat polyclonal nox2 antibody - by Bioz Stars, 2026-06
    94/100 stars

    Images

    1) Product Images from "Aldosterone promotes renal interstitial fibrosis via the AIF-1/AKT/mTOR signaling pathway"

    Article Title: Aldosterone promotes renal interstitial fibrosis via the AIF-1/AKT/mTOR signaling pathway

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2019.10680

    Effect of aldosterone on the protein expression levels of (A) AIF-1, p-PI3K, p-AKT, p-mTOR, NOX2 and Nrf2 in normal rats using western blotting. Aldosterone (0.75 µg/h subcutaneous infusion) increased the expression of (B) AIF-1, (C) p-PI3K, (D) p-AKT, (E) p-mTOR and (F) NOX2 and decreased the expression of (G) Nrf2 in normal rats, which was attenuated by spironolactone (100 mg/kg/day). *P<0.05 vs. con group, **P<0.05 vs. ald group, ***P<0.05 vs. ald + spir group. The data are presented as the mean ± standard deviation (n=3). AIF-1, allograft inflammatory factor-1; p-PI3K, phosphorylated phosphatidylinositol 3-kinase; p-AKT, phosphorylated AKT serine/threonine kinase; p-mTOR, phosphorylated mammalian target of rapamycin; NOX2, NADPH oxidase 2; Nrf2, nuclear transcription factor erythroid-related factor 2; con, control; ald, aldosterone; spir, spironolactone.
    Figure Legend Snippet: Effect of aldosterone on the protein expression levels of (A) AIF-1, p-PI3K, p-AKT, p-mTOR, NOX2 and Nrf2 in normal rats using western blotting. Aldosterone (0.75 µg/h subcutaneous infusion) increased the expression of (B) AIF-1, (C) p-PI3K, (D) p-AKT, (E) p-mTOR and (F) NOX2 and decreased the expression of (G) Nrf2 in normal rats, which was attenuated by spironolactone (100 mg/kg/day). *P<0.05 vs. con group, **P<0.05 vs. ald group, ***P<0.05 vs. ald + spir group. The data are presented as the mean ± standard deviation (n=3). AIF-1, allograft inflammatory factor-1; p-PI3K, phosphorylated phosphatidylinositol 3-kinase; p-AKT, phosphorylated AKT serine/threonine kinase; p-mTOR, phosphorylated mammalian target of rapamycin; NOX2, NADPH oxidase 2; Nrf2, nuclear transcription factor erythroid-related factor 2; con, control; ald, aldosterone; spir, spironolactone.

    Techniques Used: Expressing, Western Blot, Standard Deviation

    (A) Western blot analysis of the protein expression levels of p-PI3K, p-AKT, p-mTOR, NOX2 and Nrf2 in the rat kidney. Aldosterone (0.75 µg/h subcutaneous infusion) upregulated the expression levels of (B) p-PI3K, (C) p-AKT, (D) p-mTOR and (E) NOX2 and downregulated the expression of (F) Nrf2, which was attenuated by spironolactone (100 mg/kg/day). *P<0.05 vs. con group, **P<0.05 vs. UUO group, ***P<0.05 vs. UUO + ald group. Data are presented as the mean ± standard deviation (n=3). p-PI3K, phosphorylated phosphatidylinositol 3-kinase; p-AKT, phosphorylated AKT serine/threonine kinase; p-mTOR; phosphorylated mammalian target of rapamycin; UUO, unilateral ureteric obstruction; NOX2, NADPH oxidase 2; Nrf2, nuclear transcription factor erythroid-related factor 2; con, control; ald, aldosterone; spir, spironolactone.
    Figure Legend Snippet: (A) Western blot analysis of the protein expression levels of p-PI3K, p-AKT, p-mTOR, NOX2 and Nrf2 in the rat kidney. Aldosterone (0.75 µg/h subcutaneous infusion) upregulated the expression levels of (B) p-PI3K, (C) p-AKT, (D) p-mTOR and (E) NOX2 and downregulated the expression of (F) Nrf2, which was attenuated by spironolactone (100 mg/kg/day). *P<0.05 vs. con group, **P<0.05 vs. UUO group, ***P<0.05 vs. UUO + ald group. Data are presented as the mean ± standard deviation (n=3). p-PI3K, phosphorylated phosphatidylinositol 3-kinase; p-AKT, phosphorylated AKT serine/threonine kinase; p-mTOR; phosphorylated mammalian target of rapamycin; UUO, unilateral ureteric obstruction; NOX2, NADPH oxidase 2; Nrf2, nuclear transcription factor erythroid-related factor 2; con, control; ald, aldosterone; spir, spironolactone.

    Techniques Used: Western Blot, Expressing, Standard Deviation

    Effect of aldosterone on the protein expression levels of p-PI3K, p-AKT, p-mTOR, NOX2 and Nrf2 in RAW264.7 cells. Aldosterone (10-6 M) increased the expression levels of p-AKT, p-mTOR, NOX2 and Nrf2 via AIF-1 in RAW264.7 cells. (A) Western blot image of p-PI3K, p-AKT, p-mTOR, NOX2 and Nrf2 in different cells. Western blot analysis of (B) p-PI3K, (C) p-AKT, (D) p-mTOR, (E) NOX2 and (F) Nrf2. *P<0.05 vs. control, **P<0.05 vs. ald + pShuttle. Data are presented as the mean ± standard deviation (n=3). p-PI3K, phosphorylated phosphatidylinositol 3-kinase; p-AKT, phosphorylated AKT serine/threonine kinase; p-mTOR, phosphorylated mammalian target of rapamycin; NOX2, NADPH oxidase 2; Nrf2, nuclear transcription factor erythroid-related factor 2; AIF-1, allograft inflammatory factor-1; siRNA, small interfering RNA; over-exp, overexpression.
    Figure Legend Snippet: Effect of aldosterone on the protein expression levels of p-PI3K, p-AKT, p-mTOR, NOX2 and Nrf2 in RAW264.7 cells. Aldosterone (10-6 M) increased the expression levels of p-AKT, p-mTOR, NOX2 and Nrf2 via AIF-1 in RAW264.7 cells. (A) Western blot image of p-PI3K, p-AKT, p-mTOR, NOX2 and Nrf2 in different cells. Western blot analysis of (B) p-PI3K, (C) p-AKT, (D) p-mTOR, (E) NOX2 and (F) Nrf2. *P<0.05 vs. control, **P<0.05 vs. ald + pShuttle. Data are presented as the mean ± standard deviation (n=3). p-PI3K, phosphorylated phosphatidylinositol 3-kinase; p-AKT, phosphorylated AKT serine/threonine kinase; p-mTOR, phosphorylated mammalian target of rapamycin; NOX2, NADPH oxidase 2; Nrf2, nuclear transcription factor erythroid-related factor 2; AIF-1, allograft inflammatory factor-1; siRNA, small interfering RNA; over-exp, overexpression.

    Techniques Used: Expressing, Western Blot, Standard Deviation, Small Interfering RNA, Over Expression



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    rabbit anti rat polyclonal nox2 antibody  (Bioss)
    94
    Bioss rabbit anti rat polyclonal nox2 antibody
    Effect of aldosterone on the protein expression levels of (A) AIF-1, p-PI3K, p-AKT, p-mTOR, <t>NOX2</t> and Nrf2 in normal rats using western blotting. Aldosterone (0.75 µg/h subcutaneous infusion) increased the expression of (B) AIF-1, (C) p-PI3K, (D) p-AKT, (E) p-mTOR and (F) NOX2 and decreased the expression of (G) Nrf2 in normal rats, which was attenuated by spironolactone (100 mg/kg/day). *P<0.05 vs. con group, **P<0.05 vs. ald group, ***P<0.05 vs. ald + spir group. The data are presented as the mean ± standard deviation (n=3). AIF-1, allograft inflammatory factor-1; p-PI3K, phosphorylated phosphatidylinositol 3-kinase; p-AKT, phosphorylated AKT serine/threonine kinase; p-mTOR, phosphorylated mammalian target of rapamycin; NOX2, NADPH oxidase 2; Nrf2, nuclear transcription factor erythroid-related factor 2; con, control; ald, aldosterone; spir, spironolactone.
    Rabbit Anti Rat Polyclonal Nox2 Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti rat polyclonal nox2 antibody/product/Bioss
    Average 94 stars, based on 1 article reviews
    rabbit anti rat polyclonal nox2 antibody - by Bioz Stars, 2026-06
    94/100 stars
    		  Buy from Supplier

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    Effect of aldosterone on the protein expression levels of (A) AIF-1, p-PI3K, p-AKT, p-mTOR, NOX2 and Nrf2 in normal rats using western blotting. Aldosterone (0.75 µg/h subcutaneous infusion) increased the expression of (B) AIF-1, (C) p-PI3K, (D) p-AKT, (E) p-mTOR and (F) NOX2 and decreased the expression of (G) Nrf2 in normal rats, which was attenuated by spironolactone (100 mg/kg/day). *P<0.05 vs. con group, **P<0.05 vs. ald group, ***P<0.05 vs. ald + spir group. The data are presented as the mean ± standard deviation (n=3). AIF-1, allograft inflammatory factor-1; p-PI3K, phosphorylated phosphatidylinositol 3-kinase; p-AKT, phosphorylated AKT serine/threonine kinase; p-mTOR, phosphorylated mammalian target of rapamycin; NOX2, NADPH oxidase 2; Nrf2, nuclear transcription factor erythroid-related factor 2; con, control; ald, aldosterone; spir, spironolactone.

    Journal: Molecular Medicine Reports

    Article Title: Aldosterone promotes renal interstitial fibrosis via the AIF-1/AKT/mTOR signaling pathway

    doi: 10.3892/mmr.2019.10680

    Figure Lengend Snippet: Effect of aldosterone on the protein expression levels of (A) AIF-1, p-PI3K, p-AKT, p-mTOR, NOX2 and Nrf2 in normal rats using western blotting. Aldosterone (0.75 µg/h subcutaneous infusion) increased the expression of (B) AIF-1, (C) p-PI3K, (D) p-AKT, (E) p-mTOR and (F) NOX2 and decreased the expression of (G) Nrf2 in normal rats, which was attenuated by spironolactone (100 mg/kg/day). *P<0.05 vs. con group, **P<0.05 vs. ald group, ***P<0.05 vs. ald + spir group. The data are presented as the mean ± standard deviation (n=3). AIF-1, allograft inflammatory factor-1; p-PI3K, phosphorylated phosphatidylinositol 3-kinase; p-AKT, phosphorylated AKT serine/threonine kinase; p-mTOR, phosphorylated mammalian target of rapamycin; NOX2, NADPH oxidase 2; Nrf2, nuclear transcription factor erythroid-related factor 2; con, control; ald, aldosterone; spir, spironolactone.

    Article Snippet: The membranes were then incubated overnight at 4°C with the following primary antibodies: Rabbit anti-rat polyclonal AIF-1 (1:150; cat. no. ab153696; Abcam), rabbit anti-rat polyclonal Nrf2 antibody (1:200; cat. no. ab31163, Abcam), rabbit anti-rat polyclonal NOX2 antibody (1:300; cat. no. bs-3889R; Bioss Antibodies), rabbit anti-rat monoclonal p-PI3K p85 (1:200; cat. no. 4257; Cell Signaling Technology, Inc.), rabbit anti-rat monoclonal p-AKT (Ser473) (1:200; cat. no. 4060; Cell Signaling Technology, Inc.), rabbit anti-rat monoclonal p-mTOR (Ser2448) antibody (1:200; cat. no. #5536; Cell Signaling Technology, Inc.) and anti-β-actin monoclonal antibody (1:2,000; cat. no. A00702; GenScript).

    Techniques: Expressing, Western Blot, Standard Deviation

    (A) Western blot analysis of the protein expression levels of p-PI3K, p-AKT, p-mTOR, NOX2 and Nrf2 in the rat kidney. Aldosterone (0.75 µg/h subcutaneous infusion) upregulated the expression levels of (B) p-PI3K, (C) p-AKT, (D) p-mTOR and (E) NOX2 and downregulated the expression of (F) Nrf2, which was attenuated by spironolactone (100 mg/kg/day). *P<0.05 vs. con group, **P<0.05 vs. UUO group, ***P<0.05 vs. UUO + ald group. Data are presented as the mean ± standard deviation (n=3). p-PI3K, phosphorylated phosphatidylinositol 3-kinase; p-AKT, phosphorylated AKT serine/threonine kinase; p-mTOR; phosphorylated mammalian target of rapamycin; UUO, unilateral ureteric obstruction; NOX2, NADPH oxidase 2; Nrf2, nuclear transcription factor erythroid-related factor 2; con, control; ald, aldosterone; spir, spironolactone.

    Journal: Molecular Medicine Reports

    Article Title: Aldosterone promotes renal interstitial fibrosis via the AIF-1/AKT/mTOR signaling pathway

    doi: 10.3892/mmr.2019.10680

    Figure Lengend Snippet: (A) Western blot analysis of the protein expression levels of p-PI3K, p-AKT, p-mTOR, NOX2 and Nrf2 in the rat kidney. Aldosterone (0.75 µg/h subcutaneous infusion) upregulated the expression levels of (B) p-PI3K, (C) p-AKT, (D) p-mTOR and (E) NOX2 and downregulated the expression of (F) Nrf2, which was attenuated by spironolactone (100 mg/kg/day). *P<0.05 vs. con group, **P<0.05 vs. UUO group, ***P<0.05 vs. UUO + ald group. Data are presented as the mean ± standard deviation (n=3). p-PI3K, phosphorylated phosphatidylinositol 3-kinase; p-AKT, phosphorylated AKT serine/threonine kinase; p-mTOR; phosphorylated mammalian target of rapamycin; UUO, unilateral ureteric obstruction; NOX2, NADPH oxidase 2; Nrf2, nuclear transcription factor erythroid-related factor 2; con, control; ald, aldosterone; spir, spironolactone.

    Article Snippet: The membranes were then incubated overnight at 4°C with the following primary antibodies: Rabbit anti-rat polyclonal AIF-1 (1:150; cat. no. ab153696; Abcam), rabbit anti-rat polyclonal Nrf2 antibody (1:200; cat. no. ab31163, Abcam), rabbit anti-rat polyclonal NOX2 antibody (1:300; cat. no. bs-3889R; Bioss Antibodies), rabbit anti-rat monoclonal p-PI3K p85 (1:200; cat. no. 4257; Cell Signaling Technology, Inc.), rabbit anti-rat monoclonal p-AKT (Ser473) (1:200; cat. no. 4060; Cell Signaling Technology, Inc.), rabbit anti-rat monoclonal p-mTOR (Ser2448) antibody (1:200; cat. no. #5536; Cell Signaling Technology, Inc.) and anti-β-actin monoclonal antibody (1:2,000; cat. no. A00702; GenScript).

    Techniques: Western Blot, Expressing, Standard Deviation

    Effect of aldosterone on the protein expression levels of p-PI3K, p-AKT, p-mTOR, NOX2 and Nrf2 in RAW264.7 cells. Aldosterone (10-6 M) increased the expression levels of p-AKT, p-mTOR, NOX2 and Nrf2 via AIF-1 in RAW264.7 cells. (A) Western blot image of p-PI3K, p-AKT, p-mTOR, NOX2 and Nrf2 in different cells. Western blot analysis of (B) p-PI3K, (C) p-AKT, (D) p-mTOR, (E) NOX2 and (F) Nrf2. *P<0.05 vs. control, **P<0.05 vs. ald + pShuttle. Data are presented as the mean ± standard deviation (n=3). p-PI3K, phosphorylated phosphatidylinositol 3-kinase; p-AKT, phosphorylated AKT serine/threonine kinase; p-mTOR, phosphorylated mammalian target of rapamycin; NOX2, NADPH oxidase 2; Nrf2, nuclear transcription factor erythroid-related factor 2; AIF-1, allograft inflammatory factor-1; siRNA, small interfering RNA; over-exp, overexpression.

    Journal: Molecular Medicine Reports

    Article Title: Aldosterone promotes renal interstitial fibrosis via the AIF-1/AKT/mTOR signaling pathway

    doi: 10.3892/mmr.2019.10680

    Figure Lengend Snippet: Effect of aldosterone on the protein expression levels of p-PI3K, p-AKT, p-mTOR, NOX2 and Nrf2 in RAW264.7 cells. Aldosterone (10-6 M) increased the expression levels of p-AKT, p-mTOR, NOX2 and Nrf2 via AIF-1 in RAW264.7 cells. (A) Western blot image of p-PI3K, p-AKT, p-mTOR, NOX2 and Nrf2 in different cells. Western blot analysis of (B) p-PI3K, (C) p-AKT, (D) p-mTOR, (E) NOX2 and (F) Nrf2. *P<0.05 vs. control, **P<0.05 vs. ald + pShuttle. Data are presented as the mean ± standard deviation (n=3). p-PI3K, phosphorylated phosphatidylinositol 3-kinase; p-AKT, phosphorylated AKT serine/threonine kinase; p-mTOR, phosphorylated mammalian target of rapamycin; NOX2, NADPH oxidase 2; Nrf2, nuclear transcription factor erythroid-related factor 2; AIF-1, allograft inflammatory factor-1; siRNA, small interfering RNA; over-exp, overexpression.

    Article Snippet: The membranes were then incubated overnight at 4°C with the following primary antibodies: Rabbit anti-rat polyclonal AIF-1 (1:150; cat. no. ab153696; Abcam), rabbit anti-rat polyclonal Nrf2 antibody (1:200; cat. no. ab31163, Abcam), rabbit anti-rat polyclonal NOX2 antibody (1:300; cat. no. bs-3889R; Bioss Antibodies), rabbit anti-rat monoclonal p-PI3K p85 (1:200; cat. no. 4257; Cell Signaling Technology, Inc.), rabbit anti-rat monoclonal p-AKT (Ser473) (1:200; cat. no. 4060; Cell Signaling Technology, Inc.), rabbit anti-rat monoclonal p-mTOR (Ser2448) antibody (1:200; cat. no. #5536; Cell Signaling Technology, Inc.) and anti-β-actin monoclonal antibody (1:2,000; cat. no. A00702; GenScript).

    Techniques: Expressing, Western Blot, Standard Deviation, Small Interfering RNA, Over Expression